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Addgene

pBRC169-DXMT
(Plasmid #79822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CaMV35S- sGFP(S65T)-NOS vector
  • Backbone manufacturer
    Chiu W
  • Backbone size w/o insert (bp) 4180
  • Modifications to backbone
    cDNA fragments of CaDXMT1 (Uefuji et al. 2003) were subcloned into the BsrGI site of pBRC169 vectors by the in-fusion method (Clontech).
  • Vector type
    Plant Expression ; Reporter plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CaDXMT1
  • Alt name
    DXMT
  • Species
    Synthetic
  • Insert Size (bp)
    1155
  • Promoter CaMV 35S promoter
  • Tag / Fusion Protein
    • RC169/DsREDmonomer (169–225) (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TATATGTACAAGATGGAGCTCCAAGAAGTC
  • 3′ sequencing primer TATAGCGGCCGCTTACATGTCTGCCTTCTC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBRC169-DXMT was a gift from Yutaka Kodama & Masamitsu Wada (Addgene plasmid # 79822 ; http://n2t.net/addgene:79822 ; RRID:Addgene_79822)

  • For your References section:

    Simultaneous visualization of two protein complexes in a single plant cell using multicolor fluorescence complementation analysis. Kodama Y, Wada M. Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 10.1007/s11103-009-9467-0. Epub 2009 Feb 14. 10.1007/s11103-009-9467-0 PubMed 19219406
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