RP4-8
(Plasmid #79813)

• Purpose: Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Backbone

• Vector backbone: RP4
• Backbone manufacturer: Natural
• Backbone size w/o insert (bp): 60000
• Total vector size (bp): 60000
• Modifications to backbone: Gm gene added inserted into Tc gene by in vivo cloning. See article.
• Vector type: Helper BHR plasmid for mobilizing other plasmids carrying oriT

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): CSH56
• Growth instructions: Select for the chromosome with nalidixic acid.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gentamicin resistance gene
• Alt name: Gm
• Species: Pseudomonas - from plasmid pPH1JI
• Insert Size (bp): 800
• Promoter: Own promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NONE
• 3′ sequencing primer: NONE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We made this plasmid by in vivo recombination

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor states that users can select for the chromosome with nalidixic acid.

Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity