pLV-Golgi eGFP
(Plasmid #79809)

• Purpose: To express and target GFP into the Golgi apparatus using lentiviruses
• Depositing Lab: Pantelis Tsoulfas
• Publication: pLV-mGFP Golgi (unpublished)

Backbone

• Vector backbone: pLV MP2
• Backbone size w/o insert (bp): 6782
• Total vector size (bp): 7742
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: LB and LB agar plates in low NaCl 5gm/L
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Golgi eGFP
• Alt name: GalT1eGFP
• Alt name: UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 1 EGFP
• Species: H. sapiens (human)
• Insert Size (bp): 960
• GenBank ID: XM_005251440.4
• Entrez Gene: B4GALT1 (a.k.a. B4GAL-T1, CDG2D, CLDLFIB, GGTB2, GT1, GTB, beta4Gal-T1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Fusion of the first 62 amino acids of the human betaGalT1 gene to the eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry