pEMS1937
(Plasmid
#79782)
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PurposeHigh copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepJ204
- Total vector size (bp) 7006
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Vector typePleiades Promoter Project [sic, Pleaides Plieades]
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePle260
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Alt namePAX6 MiniPromoter
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SpeciesH. sapiens (human)
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Insert Size (bp)2147
- Promoter Ple260 (PAX6 MiniPromoter)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer pTR (5′-TGGTAGTGTGGGGACTC-3′)
- 3′ sequencing primer pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid commercially synthesized by DNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS1937 was a gift from Elizabeth Simpson (Addgene plasmid # 79782 ; http://n2t.net/addgene:79782 ; RRID:Addgene_79782) -
For your References section:
PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina. Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, Molday RS, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016. 10.1038/mtm.2016.51 PubMed 27556059