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pEMS1929
(Plasmid #79778)

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Item Catalog # Description Quantity Price (USD)
Plasmid 79778 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJ204
  • Total vector size (bp) 4785
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple256
  • Alt name
    PAX6 MiniPromoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2090
  • Promoter Ple256 (PAX6 MiniPromoter)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (unknown if destroyed)
  • 3′ cloning site See map (unknown if destroyed)
  • 5′ sequencing primer pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′)
  • 3′ sequencing primer pTR (5'-TGGTAGTGTGGGGACTC-3')
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid commercially synthesized by DNA2.0

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1929 was a gift from Elizabeth Simpson (Addgene plasmid # 79778 ; http://n2t.net/addgene:79778 ; RRID:Addgene_79778)

  • For your References section:

    PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina. Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, Molday RS, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016. 10.1038/mtm.2016.51 PubMed 27556059
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