pEMS1927
(Plasmid #79776)

• Purpose: High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert
• Depositing Lab: Elizabeth Simpson
• Publication: Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016.

Backbone

• Vector backbone: pJ204
• Total vector size (bp): 4843
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple254
• Alt name: PAX6 MiniPromoter
• Species: H. sapiens (human)
• Insert Size (bp): 2148
• Promoter: Ple254 (PAX6 MiniPromoter)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (unknown if destroyed)
• 3′ cloning site: See map (unknown if destroyed)
• 5′ sequencing primer: pTR (5′-TGGTAGTGTGGGGACTC-3′)
• 3′ sequencing primer: pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′)

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• A portion of this plasmid was derived from a plasmid made by: Plasmid commercially synthesized by DNA2.0

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA