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Addgene

hiActTS-GR
(Plasmid #79775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79775 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP c1 derivative
  • Backbone size w/o insert (bp) 3953
  • Total vector size (bp) 8204
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hiActTS-GR
  • Insert Size (bp)
    4251
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hiActTS-GR was a gift from Tetsuya Kitaguchi (Addgene plasmid # 79775 ; http://n2t.net/addgene:79775 ; RRID:Addgene_79775)

  • For your References section:

    Wide and high resolution tension measurement using FRET in embryo. Yamashita S, Tsuboi T, Ishinabe N, Kitaguchi T, Michiue T. Sci Rep. 2016 Jun 23;6:28535. doi: 10.1038/srep28535. 10.1038/srep28535 PubMed 27335157
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