MAPK3_HUMAN_D0
(Plasmid #79702)

• Purpose: This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
• Depositing Labs: John Chodera, Nicholas Levinson, Markus Seeliger
• Publication: Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.

Backbone

• Vector backbone: pET His10 TEV LIC cloning vector (2B-T-10)
• Backbone manufacturer: Scott Gradia (Addgene plasmid # 78173)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MAPK3
• Species: H. sapiens (human)
• Entrez Gene: MAPK3 (a.k.a. ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK)
• Promoter: T7
• Tag / Fusion Protein:
  • His10-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated as part of an open library of human kinase domain constructs for automated bacterial expression. The work can be found here doi: http://dx.doi.org/10.1101/038711 The lambda phosphatase expression vector can be found at https://www.addgene.org/79748/ The YopH Phosphatase expression vector can be found at https://www.addgene.org/79749/