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Addgene

5HT2C-RNA1-FLAG
(Plasmid #79678)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5HT2C-RNA1
  • Alt name
    serotonin receptor 2C short isoform
  • Alt name
    HTR2C, RNA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    HTR2C (a.k.a. 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C)
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5HT2C-RNA1-FLAG was a gift from Stefan Stamm (Addgene plasmid # 79678 ; http://n2t.net/addgene:79678 ; RRID:Addgene_79678)

  • For your References section:

    Oligonucleotide-induced alternative splicing of serotonin 2C receptor reduces food intake. Zhang Z, Shen M, Gresch PJ, Ghamari-Langroudi M, Rabchevsky AG, Emeson RB, Stamm S. EMBO Mol Med. 2016 Aug 1;8(8):878-94. doi: 10.15252/emmm.201506030. 10.15252/emmm.201506030 PubMed 27406820
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