Farnesylated SE pcDNA3.1
(Plasmid
#79666)
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PurposeExpresses super ecliptic A227D in mammalian cell membranes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 6377
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesuperEcliptic pHluorin
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Insert Size (bp)714
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Farnesylated SE pcDNA3.1 was a gift from Bradley Baker (Addgene plasmid # 79666 ; http://n2t.net/addgene:79666 ; RRID:Addgene_79666) -
For your References section:
Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions. Kang BE, Baker BJ. Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865. 10.1038/srep23865 PubMed 27040905