pEMS2046
(Plasmid
#79654)
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PurposessAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonessAAV2
- Backbone size w/o insert (bp) 4972
- Total vector size (bp) 7118
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)SURE
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namessAAV Ple257-EmGFP WPRE
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Alt namePle257 (PAX6 Enhancer)
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SpeciesH. sapiens (human)
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Insert Size (bp)2160
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GenBank ID
- Promoter PAX6 Enhancer
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer M13pUC-rev
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2046 was a gift from Elizabeth Simpson (Addgene plasmid # 79654 ; http://n2t.net/addgene:79654 ; RRID:Addgene_79654) -
For your References section:
PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina. Hickmott JW, Chen CY, Arenillas DJ, Korecki AJ, Lam SL, Molday LL, Bonaguro RJ, Zhou M, Chou AY, Mathelier A, Boye SL, Hauswirth WW, Molday RS, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2016 Aug 10;3:16051. doi: 10.1038/mtm.2016.51. eCollection 2016. 10.1038/mtm.2016.51 PubMed 27556059