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Addgene

GALTA-c001
(Plasmid #79645)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79645 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    SGC; Addgene Plasmid #26103
  • Backbone size w/o insert (bp) 7284
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GALT
  • Alt name
    galactose-1-phosphate uridylyltransferase
  • Alt name
    PDB ID: 5IN3
  • Species
    H. sapiens (human)
  • Entrez Gene
    GALT
  • Promoter T7
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21(DE3)-R3-pRARE2 or other strain with T7 RNA polymerase for protein expression. For more information, please see: http://www.thesgc.org/structures/5IN3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GALTA-c001 was a gift from Nicola Burgess-Brown (Addgene plasmid # 79645 ; http://n2t.net/addgene:79645 ; RRID:Addgene_79645)