GALTA-c001
(Plasmid #79645)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC 2016 plasmids (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC; Addgene Plasmid #26103
• Backbone size w/o insert (bp): 7284
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GALT
• Alt name: galactose-1-phosphate uridylyltransferase
• Alt name: PDB ID: 5IN3
• Species: H. sapiens (human)
• Entrez Gene: GALT
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use BL21(DE3)-R3-pRARE2 or other strain with T7 RNA polymerase for protein expression. For more information, please see: http://www.thesgc.org/structures/5IN3