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Addgene

JARID1BA-c539
(Plasmid #79643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFB-LIC-Bse
  • Backbone manufacturer
    SGC, Addgene Plasmid #26108
  • Backbone size w/o insert (bp) 6816
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JARID1B
  • Alt name
    KDM5B
  • Alt name
    Jumonji, AT rich interactive domain 1B (RBP2-like); putative DNA/chromatin binding motif
  • Alt name
    PDB ID: 5FV3
  • Species
    H. sapiens (human)
  • Entrez Gene
    KDM5B (a.k.a. CT31, JARID1B, MRT65, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1)
  • Promoter polyhedrin
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer fBac-1 (5'-TATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer fBac-2 (5'-GGGAGGTTTTTTAAAGCAAGTAAA-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use SF9 for protein expression. For more information, please see: http://www.thesgc.org/structures/5FV3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JARID1BA-c539 was a gift from Nicola Burgess-Brown (Addgene plasmid # 79643 ; http://n2t.net/addgene:79643 ; RRID:Addgene_79643)
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