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pAGM_Puro_shRNA n.2_mTet3
(Plasmid #79625)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM
  • Backbone size w/o insert (bp) 7581
  • Total vector size (bp) 7678
  • Modifications to backbone
    GFP has been substituted with a Puromycin selection cassette
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tet3
  • gRNA/shRNA sequence
    5'-TCCAACGAGAAGCTATTTT
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_183138.2
  • Entrez Gene
    Tet3 (a.k.a. B430006D22Rik, D230004J03Rik)
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5'- GGTTTATTACAGGGACAGCAGAG-3'
  • 3′ sequencing primer 5'-CCACCGGTATCGATACGCGTC-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM_Puro_shRNA n.2_mTet3 was a gift from Silvia Monticelli (Addgene plasmid # 79625 ; http://n2t.net/addgene:79625 ; RRID:Addgene_79625)

  • For your References section:

    TET2 Regulates Mast Cell Differentiation and Proliferation through Catalytic and Non-catalytic Activities. Montagner S, Leoni C, Emming S, Della Chiara G, Balestrieri C, Barozzi I, Piccolo V, Togher S, Ko M, Rao A, Natoli G, Monticelli S. Cell Rep. 2016 May 17;15(7):1566-79. doi: 10.1016/j.celrep.2016.04.044. Epub 2016 May 5. 10.1016/j.celrep.2016.04.044 PubMed 27160912
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