pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT) Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT) with the following enzymes: NheI-HF and HindIII-HF.
Expected Bands
NheI-HF: 10,167 bp
HindIII-HF: 6999 bp, 1833 bp, 509 bp, 387 bp, and 366 bp
We observe the expected bands for this digest.
