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Addgene

MO70-mJP-4
(Plasmid #79598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MO70
  • Backbone size w/o insert (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Junctophilin-4
  • Alt name
    Jph4
  • Alt name
    NM_177049.5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1886
  • Entrez Gene
    Jph4 (a.k.a. 9330157P13Rik, JP-4, JPHL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA encoding murine Junctophilin-4 (JP4, clone ID 6810400) was purchased from Open Biosystems (GE Dharmacon)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MO70-mJP-4 was a gift from Yousang Gwack (Addgene plasmid # 79598 ; http://n2t.net/addgene:79598 ; RRID:Addgene_79598)

  • For your References section:

    Junctophilin-4, a component of the endoplasmic reticulum-plasma membrane junctions, regulates Ca2+ dynamics in T cells. Woo JS, Srikanth S, Nishi M, Ping P, Takeshima H, Gwack Y. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2762-7. doi: 10.1073/pnas.1524229113. Epub 2016 Feb 29. 10.1073/pnas.1524229113 PubMed 26929330
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