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p-mCherry-C1-hCRACR2A-isoform-a
(Plasmid #79593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRACR2A-isoform-a
  • Alt name
    EFCAB4B
  • Alt name
    NM_001144958
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2196
  • Entrez Gene
    CRACR2A (a.k.a. EFCAB4B, RAB46)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer mCherry-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-mCherry-C1-hCRACR2A-isoform-a was a gift from Yousang Gwack (Addgene plasmid # 79593 ; http://n2t.net/addgene:79593 ; RRID:Addgene_79593)

  • For your References section:

    A large Rab GTPase encoded by CRACR2A is a component of subsynaptic vesicles that transmit T cell activation signals. Srikanth S, Kim KD, Gao Y, Woo JS, Ghosh S, Calmettes G, Paz A, Abramson J, Jiang M, Gwack Y. Sci Signal. 2016 Mar 22;9(420):ra31. doi: 10.1126/scisignal.aac9171. 10.1126/scisignal.aac9171 PubMed 27016526
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