MO70-N-Flag-hCRACR2A-isoform-a
(Plasmid #79592)

• Purpose: Transient and retroviral expression
• Depositing Lab: Yousang Gwack
• Publication: Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi: 10.1126/scisignal.aac9171.

Backbone

• Vector backbone: MO70
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRACR2A-isoform-a
• Alt name: EFCAB4B
• Alt name: NM_001144958
• Species: H. sapiens (human)
• Insert Size (bp): 2196
• Entrez Gene: CRACR2A (a.k.a. EFCAB4B, RAB46)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pCDH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This cDNA was cloned from a cDNA library made from Jurkat T cells, as described in the reference