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MO70-hCRACR2A-isoform-c
(Plasmid #79591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MO70
  • Backbone size w/o insert (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRACR2A-isoform-c
  • Alt name
    EFCAB4B
  • Alt name
    NM_032680
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1188
  • Entrez Gene
    CRACR2A (a.k.a. EFCAB4B, RAB46)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original clone containing this cDNA was obtained from open biosystems

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MO70-hCRACR2A-isoform-c was a gift from Yousang Gwack (Addgene plasmid # 79591 ; http://n2t.net/addgene:79591 ; RRID:Addgene_79591)

  • For your References section:

    A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Srikanth S, Jung HJ, Kim KD, Souda P, Whitelegge J, Gwack Y. Nat Cell Biol. 2010 May;12(5):436-46. doi: 10.1038/ncb2045. Epub 2010 Apr 25. 10.1038/ncb2045 PubMed 20418871
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