CIBN-CAAX
(Plasmid #79574)

• Purpose: expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
• Depositing Labs: Pietro De Camilli, Olof Idevall-Hagren
• Publication: Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30.

Backbone

• Vector backbone: CIBN-GFP-CAAX
• Backbone manufacturer: C. Tucker, University of Colorado Denver
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIBN
• Alt name: Cry2 binding domain
• Species: A. thaliana (mustard weed)
• Mutation: K93A, R94A, K106A, K107A (please see depositor comments below)
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: CMV
• Tag / Fusion Protein:
  • C-terminal CAAX-box (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutations K93A, R94A, K106A, K107A are mutations of putative nuclear localisation signals in CIB1.