CIBN-GFP-TMD
(Plasmid #79571)

• Purpose: expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
• Depositing Labs: Pietro De Camilli, Olof Idevall-Hagren
• Publication: Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30.

Backbone

• Vector backbone: pEGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: syntaxin 1A
• Species: M. musculus (mouse)
• Mutation: aa 249_288 (C-terminal region (polybasic stretch and transmembrane domain from human Syntaxin 1A)
• Entrez Gene: Stx1a (a.k.a. HPC-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • CIBN (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PvuI (not destroyed)
• 3′ cloning site: BsrG1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the C-terminal region (polybasic stretch and transmembrane domain; amino acids 249-288) from human Syntaxin 1A (NM_004603.3).