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Addgene

GFP-CRY2-P14P5K
(Plasmid #79569)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pip5k1c
  • Species
    M. musculus (mouse)
  • Mutation
    R445E and R446E; truncation of amino acids 636-661 (please see depositor comments below)
  • Entrez Gene
    Pip5k1c (a.k.a. AI115456, AI835305, Pip5kIgamma)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • CRY2 (photolyase homology region domain of Arabidopsis thaliana cryptochrome 2) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Three modifications have been made to PIP5K1g (NM008844): R445E and R446E mutations to favour cytosolic distribution and truncation of amino acids 636-661 to prevent plasma membrane localisation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-CRY2-P14P5K was a gift from Olof Idevall-Hagren (Addgene plasmid # 79569 ; http://n2t.net/addgene:79569 ; RRID:Addgene_79569)

  • For your References section:

    Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Regulates Ca(2+)-Influx and Insulin Secretion from Pancreatic beta Cells. Xie B, Nguyen PM, Gucek A, Thonig A, Barg S, Idevall-Hagren O. Cell Chem Biol. 2016 Jul 21;23(7):816-26. doi: 10.1016/j.chembiol.2016.06.009. 10.1016/j.chembiol.2016.06.009 PubMed 27447049
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