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Addgene

CRY2-OCRL
(Plasmid #79566)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OCRL
  • Species
    H. sapiens (human)
  • Mutation
    aa 234_539
  • Entrez Gene
    OCRL (a.k.a. DENT2, Dent-2, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • CRY2 (photolyase homology region domain of Arabidopsis thaliana cryptochrome 2) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2-OCRL was a gift from Olof Idevall-Hagren (Addgene plasmid # 79566 ; http://n2t.net/addgene:79566 ; RRID:Addgene_79566)
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