pNR291
(Plasmid
#79555)
-
PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBAC
-
Backbone manufacturerWild, J.; Hradecna, Z.; Szybalski, W. Genome Res. 2002 Sep; 12(9): 1434–1444. DOI: 10.1101/gr.130502
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert 1
-
Gene/Insert nameGFPmut3b
Gene/Insert 2
-
Gene/Insert namemTagBFP
Gene/Insert 3
-
Gene/Insert namemRFP
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pNR291 was a gift from Timothy Lu (Addgene plasmid # 79555 ; http://n2t.net/addgene:79555 ; RRID:Addgene_79555) -
For your References section:
Synthetic recombinase-based state machines in living cells. Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK. Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21. 10.1126/science.aad8559 PubMed 27463678
Map uploaded by the depositor.
