pNR220
(Plasmid #79541)

• Purpose: Input plasmid: expresses three serine recombinases downstream of three inducible promoters.
• Depositing Lab: Timothy Lu
• Publication: Roquet et al Science. 2016 Jul 22;353(6297):aad8559. doi: 10.1126/science.aad8559. Epub 2016 Jul 21.

Backbone

• Vector backbone: Dual-Recombinase-Controller
• Backbone manufacturer: Addgene Plasmid #44456
• Total vector size (bp): 9435
• Modifications to backbone: Replaced CamR with KanR.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alphaPRO
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TP901
• Tags / Fusion Proteins:
  • His tag (N terminal on insert)
  • LAA degradation tag (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: BxbI

Gene/Insert 3

• Gene/Insert name: A118

Resource Information

• Supplemental Documents:
  • GenBank
• A portion of this plasmid was derived from a plasmid made by: The plasmid is derived from the Dual-Recombinase-Controller (addgene plasmid 44456) from the Endy Lab, modified to have KanR instead of CamR on the backbone, and also with and added recombinase (A118) and inducible promoter system (PhlF) to drive it.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The DH5alphaPRO strain is DH5alpha modified to express LacI and TetR constitutively.