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5CZG (Tb427.10.7420)
(Plasmid #79517)

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Item Catalog # Description Quantity Price (USD)
Plasmid 79517 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET15-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #26092)
  • Backbone size w/o insert (bp) 7737
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tb427.10.7420
  • Alt name
    Tb427.10.7420:MAC054-G03:C234711
  • Alt name
    Tb427.10.7420:M1-D115:G3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    345
  • Promoter T7-lacO
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see http://www.thesgc.org/structures/5DZC for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5CZG (Tb427.10.7420) was a gift from Cheryl Arrowsmith (Addgene plasmid # 79517 ; http://n2t.net/addgene:79517 ; RRID:Addgene_79517)
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