pENTR221-JUN
(Plasmid
#79512)
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PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR221-P5P2
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 4774
- Total vector size (bp) 3653
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Modifications to backboneGateway cloned JUN Insert
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Vector typeMultisite Gateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJUN
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SpeciesH. sapiens (human)
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Insert Size (bp)1080
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GenBank ID
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Entrez GeneJUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
- Promoter no Promoter
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Tag
/ Fusion Protein
- HA Tag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-FP
- 3′ sequencing primer M13-RP (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multisite Gateway Entry Clone for HA tagged JUN CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221-JUN was a gift from Peter Jon Nelson (Addgene plasmid # 79512 ; http://n2t.net/addgene:79512 ; RRID:Addgene_79512) -
For your References section:
A vector platform for the rapid and efficient engineering of stable complex transgenes. Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ. Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365. 10.1038/srep34365 PubMed 27694838
