pENTR221-YAP1 CA
(Plasmid #79502)

• Purpose: MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS
• Depositing Lab: Peter Jon Nelson
• Publication: Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365.

Backbone

• Vector backbone: pDONR221-P5P2
• Backbone manufacturer: Thermo Scientific
• Backbone size w/o insert (bp): 4774
• Total vector size (bp): 4132
• Modifications to backbone: Gateway cloned YAP1 S127A Insert
• Vector type: Multisite Gateway Entry Clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP1
• Species: H. sapiens (human)
• Insert Size (bp): 1545
• Mutation: S127A
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: no Promoter
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13-FP
• 3′ sequencing primer: M13-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Multisite Gateway Entry Clone for human YAP1 S127A Constitutively Active mutant CDS. Second Fragment for 2 Fragment MultiSite Gateway LR reaction.