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pENTR221-PGK.Pro
(Plasmid #79489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221-P1P5r
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 4765
  • Total vector size (bp) 3242
  • Modifications to backbone
    Gateway cloned PGK Promoter Insert
  • Vector type
    Multisite Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGK Promoter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5333
  • Entrez Gene
    PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)
  • Promoter PGK

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multisite Gateway Entry Clone for human PGK Promoter. First Fragment for 2 Fragment MultiSite Gateway LR reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR221-PGK.Pro was a gift from Peter Jon Nelson (Addgene plasmid # 79489 ; http://n2t.net/addgene:79489 ; RRID:Addgene_79489)

  • For your References section:

    A vector platform for the rapid and efficient engineering of stable complex transgenes. Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ. Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365. 10.1038/srep34365 PubMed 27694838
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