pSBDEST.IP
(Plasmid
#79472)
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Purpose(Empty Backbone) Promoterless, Sleeping Beauty-compatible Gateway destination vector, IRES Puromycin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSBDEST.IP
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Backbone manufacturercloned in house
- Backbone size (bp) 7294
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Vector typePromoterless Gateway Destination vector
- Promoter none
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer pIRES-RP (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning. Uses an internal ribosomal entry site to tie Puromycin selection to expression of inserted transgene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBDEST.IP was a gift from Peter Jon Nelson (Addgene plasmid # 79472 ; http://n2t.net/addgene:79472 ; RRID:Addgene_79472) -
For your References section:
A vector platform for the rapid and efficient engineering of stable complex transgenes. Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ. Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365. 10.1038/srep34365 PubMed 27694838
