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pLNHA-C1-Hs4E-BP3
(Plasmid #79439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLNHA-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4138
  • Total vector size (bp) 4177
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hs4E-BP3
  • Alt name
    EIF4EBP3
  • Alt name
    eukaryotic initiation factor 4E-binding protein 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    303
  • GenBank ID
    NM_003732
  • Entrez Gene
    EIF4EBP3 (a.k.a. 4E-BP3, 4EBP3)
  • Promoter CMV
  • Tag / Fusion Protein
    • lambdaN-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-forw
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNHA-C1-Hs4E-BP3 was a gift from Elisa Izaurralde (Addgene plasmid # 79439 ; http://n2t.net/addgene:79439 ; RRID:Addgene_79439)

  • For your References section:

    Molecular architecture of 4E-BP translational inhibitors bound to eIF4E. Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, Weichenrieder O, Izaurralde E. Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19. 10.1016/j.molcel.2015.01.017 PubMed 25702871
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