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Addgene

pGEX-4T-2/hGGA3(GAT)
(Plasmid #79436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-2
  • Backbone manufacturer
    GE Healthcare
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GGA3
  • Species
    H. sapiens (human)
  • Mutation
    amino acids 107-286
  • GenBank ID
    NP_054720.1.
  • Entrez Gene
    GGA3
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-2/hGGA3(GAT) was a gift from Kazuhisa Nakayama (Addgene plasmid # 79436 ; http://n2t.net/addgene:79436 ; RRID:Addgene_79436)

  • For your References section:

    GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors. Takatsu H, Yoshino K, Toda K, Nakayama K. Biochem J. 2002 Jul 15;365(Pt 2):369-78. 10.1042/BJ20020428 PubMed 11950392
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