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Addgene

pcDNA3/hArf5(T31N)-HA
(Plasmid #79429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arf5
  • Species
    H. sapiens (human)
  • Mutation
    changed Threonine 31 to Asparagine
  • Entrez Gene
    ARF5
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/hArf5(T31N)-HA was a gift from Kazuhisa Nakayama (Addgene plasmid # 79429 ; http://n2t.net/addgene:79429 ; RRID:Addgene_79429)

  • For your References section:

    Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. Hanai A, Ohgi M, Yagi C, Ueda T, Shin HW, Nakayama K. J Biochem. 2016 Feb;159(2):201-8. doi: 10.1093/jb/mvv088. Epub 2015 Sep 1. 10.1093/jb/mvv088 PubMed 26330566
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