pcDNA3/hArf5(Q71L)-HA
(Plasmid
#79428)
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PurposeExpresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArf5
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SpeciesH. sapiens (human)
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Mutationchanged Glutamine 71 to Leucine
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Entrez GeneARF5
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3/hArf5(Q71L)-HA was a gift from Kazuhisa Nakayama (Addgene plasmid # 79428 ; http://n2t.net/addgene:79428 ; RRID:Addgene_79428) -
For your References section:
Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. Hanai A, Ohgi M, Yagi C, Ueda T, Shin HW, Nakayama K. J Biochem. 2016 Feb;159(2):201-8. doi: 10.1093/jb/mvv088. Epub 2015 Sep 1. 10.1093/jb/mvv088 PubMed 26330566