-
PurposeExpresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 79422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameArf6
-
SpeciesH. sapiens (human)
-
Entrez GeneARF6
-
Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3/hArf6(WT)-mCherry was a gift from Kazuhisa Nakayama (Addgene plasmid # 79422 ; http://n2t.net/addgene:79422 ; RRID:Addgene_79422) -
For your References section:
Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. Makyio H, Ohgi M, Takei T, Takahashi S, Takatsu H, Katoh Y, Hanai A, Ueda T, Kanaho Y, Xie Y, Shin HW, Kamikubo H, Kataoka M, Kawasaki M, Kato R, Wakatsuki S, Nakayama K. EMBO J. 2012 May 30;31(11):2590-603. doi: 10.1038/emboj.2012.89. Epub 2012 Apr 20. 10.1038/emboj.2012.89 PubMed 22522702