pCAG/hArf4(WT)-EGFP
(Plasmid
#79407)
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PurposeExpresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG/hArf4(WT)-EGFP was a gift from Kazuhisa Nakayama (Addgene plasmid # 79407 ; http://n2t.net/addgene:79407 ; RRID:Addgene_79407) -
For your References section:
ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HW. Mol Biol Cell. 2013 Aug;24(16):2570-81. doi: 10.1091/mbc.E13-04-0197. Epub 2013 Jun 19. 10.1091/mbc.E13-04-0197 PubMed 23783033