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Addgene

pCAG/hArf4(T31N)-HA
(Plasmid #79405)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arf4
  • Species
    H. sapiens (human)
  • Mutation
    T31N
  • Entrez Gene
    ARF4 (a.k.a. ARF2)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG/hArf4(T31N)-HA was a gift from Kazuhisa Nakayama (Addgene plasmid # 79405 ; http://n2t.net/addgene:79405 ; RRID:Addgene_79405)

  • For your References section:

    ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HW. Mol Biol Cell. 2013 Aug;24(16):2570-81. doi: 10.1091/mbc.E13-04-0197. Epub 2013 Jun 19. 10.1091/mbc.E13-04-0197 PubMed 23783033
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