Skip to main content
Addgene

pAc5.1B-EGFP-Dm4E-T
(Plasmid #79261)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6071
  • Total vector size (bp) 6089
  • Vector type
    Schneider 2 cell Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dm4E-T
  • Alt name
    CG32016C
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3033
  • GenBank ID
  • Entrez Gene
    4E-T (a.k.a. Dmel_CG32016, CG11097, CG11232, CG32016, Dm4E-T, Dmel\CG32016)
  • Promoter Ac5 promoter
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh rev primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-Dm4E-T was a gift from Elisa Izaurralde (Addgene plasmid # 79261 ; http://n2t.net/addgene:79261 ; RRID:Addgene_79261)

  • For your References section:

    4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation. Igreja C, Peter D, Weiler C, Izaurralde E. Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790. 10.1038/ncomms5790 PubMed 25179781
Related items:
Commonly requested with: