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pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
(Plasmid #79259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-lambdaN-HA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5489
  • Total vector size (bp) 5492
  • Vector type
    Schneider 2 cell Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmeIF4G
  • Alt name
    eukaryotic translation initiation factor 4G
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    4998
  • GenBank ID
    NM_079901
  • Entrez Gene
    eIF4G1 (a.k.a. Dmel_CG10811, CG10811, Dmel\CG10811, EIF4G, EIF4G1, Eif4G, deIF4G, eIF-4G, eIF4G, eIF4g, eiF-4G, p200)
  • Promoter Ac5 promoter
  • Tags / Fusion Proteins
    • lambdaN-HA (N terminal on backbone)
    • V5-His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Ac5 primer
  • 3′ sequencing primer Bgh rev primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-lambdaN-HA-DmeIF4G-V5His6 was a gift from Elisa Izaurralde (Addgene plasmid # 79259 ; http://n2t.net/addgene:79259 ; RRID:Addgene_79259)

  • For your References section:

    GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation. Zekri L, Kuzuoglu-Ozturk D, Izaurralde E. EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 2013 Mar 5. 10.1038/emboj.2013.44 PubMed 23463101
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