pAc5.1B-EGFP-DmPAN3
(Plasmid #79257)

• Purpose: Expresses EGFP-tagged DmPAN3 in S2 cells
• Depositing Lab: Elisa Izaurralde
• Publication: Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Backbone

• Vector backbone: pAc5.1B-EGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6070
• Total vector size (bp): 6089
• Vector type: Schneider 2 cell Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PAN3
• Species: D. melanogaster (fly)
• Insert Size (bp): 2373
• GenBank ID: NM_139510
• Entrez Gene: CG11486 (a.k.a. Dmel_CG11486, Dmel\CG11486, PAN3)
• Promoter: Ac5 promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-forw
• 3′ sequencing primer: Bgh rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry