pAc5.1B-EGFP-DmNot2
(Plasmid #79255)

• Purpose: Expresses EGFP-tagged DmNot2 in S2 cells
• Depositing Lab: Elisa Izaurralde
• Publication: Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302.

Backbone

• Vector backbone: pAc5.1B-EGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6051
• Total vector size (bp): 6089
• Vector type: Schneider 2 cell Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmNot2
• Alt name: regena
• Species: D. melanogaster (fly)
• Insert Size (bp): 1758
• GenBank ID: NM_169080
• Entrez Gene: Rga (a.k.a. Dmel_CG2161, CG2138, CG2161, Dmel\CG2161, NOT2, Not2, RGA, l(3)03834, not2)
• Promoter: Ac5 promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-forw
• 3′ sequencing primer: Bgh rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry