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pAc5.1B-EGFP-DmNot10
(Plasmid #79254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6066
  • Total vector size (bp) 6089
  • Vector type
    Schneider 2 cell Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmNot10
  • Alt name
    CG18616
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1908
  • GenBank ID
    NM_141958
  • Entrez Gene
    Not10 (a.k.a. Dmel_CG18616, CG18616, Dmel\CG18616, NOT10, dNot10)
  • Promoter Ac5 promoter
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh rev primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmNot10 was a gift from Elisa Izaurralde (Addgene plasmid # 79254 ; http://n2t.net/addgene:79254 ; RRID:Addgene_79254)

  • For your References section:

    NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain. Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E. RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 Jan 9. 10.4161/rna.23018 PubMed 23303381
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