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pAc5.1B-EGFP-DmCCr4
(Plasmid #79252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Schneider 2 cell Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmCaf40
  • Alt name
    twin
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    -1
  • GenBank ID
    NM_170129
  • Entrez Gene
    twin (a.k.a. Dmel_CG31137, BEST:GH20274, CCR4, CG17741, CG31137, CG5534, CT39331, Ccr4, Dmel\CG31137, Twin, ccr4, twn)
  • Promoter Ac5 promoter
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh rev primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmCCr4 was a gift from Elisa Izaurralde (Addgene plasmid # 79252 ; http://n2t.net/addgene:79252 ; RRID:Addgene_79252)

  • For your References section:

    HPat provides a link between deadenylation and decapping in metazoa. Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E. J Cell Biol. 2010 Apr 19. 189(2):289-302. 10.1083/jcb.200910141 PubMed 20404111
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