pAc5.1B-EGFP-DmCaf40
(Plasmid
#79251)
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PurposeExpresses EGFP-tagged DmCaf40 in S2 cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79251 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAc5.1B-EGFP
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6057
- Total vector size (bp) 6089
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Vector typeSchneider 2 cell Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDmCaf40
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Alt nameRcd
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SpeciesD. melanogaster (fly)
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Insert Size (bp)915
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GenBank IDNM_167675
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Entrez GeneRcd-1 (a.k.a. Dmel_CG14213, CAF40, CG14213, Caf40, Dmel\CG14213, Drcd-1)
- Promoter Ac5 promoter
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EGFP-forw
- 3′ sequencing primer Bgh rev primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1B-EGFP-DmCaf40 was a gift from Elisa Izaurralde (Addgene plasmid # 79251 ; http://n2t.net/addgene:79251 ; RRID:Addgene_79251) -
For your References section:
NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain. Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E. RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 Jan 9. 10.4161/rna.23018 PubMed 23303381