pAc5.1B-EGFP-DmNot3
(Plasmid #79250)

• Purpose: Expresses EGFP-tagged DmNot3 in S2 cells
• Depositing Lab: Elisa Izaurralde
• Publication: Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302.

Backbone

• Vector backbone: pAc5.1B-EGFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6065
• Total vector size (bp): 6089
• Modifications to backbone: N-terminal EGFP-tag
• Vector type: Schneider 2 cell Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmNot3
• Alt name: CG8426
• Species: D. melanogaster (fly)
• Insert Size (bp): 2535
• GenBank ID: NM_136332
• Entrez Gene: Not3 (a.k.a. Dmel_CG8426, CG8426, CNOT3, Dmel\CG8426, NOT3, NOT3.5, NOT3/5, l(2)NC136, not3, not3/5)
• Promoter: Ac5 promoter
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-forw
• 3′ sequencing primer: Bgh rev primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry