MSCV-(pBabe mcs)- human p210BCR-ABL-IRES-GFP
(Plasmid #79248)

• Purpose: retroviral mammalian expression vector for human p210BCR-ABL and GFP
• Depositing Lab: Martine Roussel
• Publication: Roussel lab plasmids (unpublished)

Backbone

• Vector backbone: MSCV
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p210 BCR-ABL
• Species: H. sapiens (human)
• Mutation: T1065S
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN5'
• 3′ sequencing primer: pCDH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the p210BCR-ABL cDNA came from Dr Owen Witte, UCLA
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry