MSCV-(pBabe mcs)-human p210BCR-ABL-IRES-Luc2
(Plasmid
#79247)
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Purposeretroviral mammalian expression vector for human p210BCR-ABL and luciferase
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMSCV
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep210 BCR-ABL
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SpeciesH. sapiens (human)
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Entrez GeneABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
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Tag
/ Fusion Protein
- IRES-luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bythe p210BCR-ABL cDNA came from Dr Owen Witte, UCLA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-(pBabe mcs)-human p210BCR-ABL-IRES-Luc2 was a gift from Martine Roussel (Addgene plasmid # 79247 ; http://n2t.net/addgene:79247 ; RRID:Addgene_79247)
Map uploaded by the depositor.
