pGEX-4T-3-mR7BD
(Plasmid #79149)

• Purpose: bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)
• Depositing Lab: Aimee Edinger
• Publication: Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40. doi: 10.1091/mbc.E08-09-0911. Epub 2009 Apr 22.

Backbone

• Vector backbone: pGEX-4T-3
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 5100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: R7BD
• Alt name: RILP
• Alt name: Rab interacting lysosomal protein
• Species: M. musculus (mouse)
• Insert Size (bp): 240
• Mutation: amino acids 220-299
• GenBank ID: NM_001029938.2
• Entrez Gene: Rilp (a.k.a. Gm857)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Supplemental Documents:
  • GST fusion protein purification
  • GST pulldown assay
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry