pEF6-myc-TBC1D15
(Plasmid #79148)

• Purpose: mammalian expression of TBC1D15
• Depositing Lab: Aimee Edinger
• Publication: Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-21.

Backbone

• Vector backbone: pEF6/V5-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TBC1D15
• Alt name: TBC1 domain family, member 15
• Species: M. musculus (mouse)
• Mutation: S616N (please see depositor comment below)
• Entrez Gene: Tbc1d15 (a.k.a. 4432405K22Rik, AU022358, AU023730, AW557774, Ly6dl, Rab7-GAP)
• Promoter: EF-1alpha
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • V5 (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note- The TBC1D15 mutation S616N identified during Addgene's quality control process does not affect plasmid function.