pMpGWB103-Citrine
(Plasmid
#79137)
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PurposeBinary vectors for expression of Citrine fluorescent protein in M. polymorpha
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMpGWB103
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 11282
- Total vector size (bp) 11999
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCitrine
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Insert Size (bp)717
- Promoter MpEF1a promoter
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Tag
/ Fusion Protein
- Citrine
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGTGAGCAAGGGCGAG
- 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACTTGTACAGCTCGTCC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMpGWB103-Citrine was a gift from Yutaka Kodama (Addgene plasmid # 79137 ; http://n2t.net/addgene:79137 ; RRID:Addgene_79137) -
For your References section:
AgarTrap: a simplified Agrobacterium-mediated transformation method for sporelings of the liverwort Marchantia polymorpha L. Tsuboyama S, Kodama Y. Plant Cell Physiol. 2014 Jan;55(1):229-36. doi: 10.1093/pcp/pct168. Epub 2013 Nov 19. 10.1093/pcp/pct168 PubMed 24259681
Map uploaded by the depositor.
